美國Applied Biosystems? 7500實時熒光定量PCR系統
美國Applied Biosystems? 7500實時熒光定量PCR系統 | 時間: December-25 14:54:34 | 分類:其它儀器
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7500實時熒光定量PCR系統描述
The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This system is a sophisticated platform for users who require extended capabilities and maximum versatility. The 3rd generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.
• Powerful, five-color platform is calibrated for the broadest range of dyes available: FAM™⁄SYBR® Green I, VIC®⁄JOE™, NED™⁄ TAMRA™⁄ Cy3™, ROX™⁄Texas Red®, and Cy5™ dyes
• Specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets
• Advanced multi-componenting algorithm minimizes spectral crosstalk – superior for multiplexing
• User-friendly software includes plate set-up wizards, multi-plate data viewing capabilities, and advanced analysis tools to make data processing simple and straightforward
NEW - 7500 Software v2.0
Now the easy-to-use StepOne software is available for both the 7500 and 7500 Fast systems with the 7500 Software v2.0 upgrade. The 7500 Software v2.0 incorporates your favorite StepOne Software features, such as a variety of plate setup wizards, standard curve dilution and master mix recipe calculators, QC flags, data filters, and email notification when a run is finished. The 7500 Software v2.0 also includes an enhanced Gene Expression Study package and has a variety of new melting curve protocol options, including multiple peak detection, step and hold temperature control, and customizable ramp rates.
The NEW Gene Expression Study package accommodates large studies better than any other instrument software package:
• Import an unlimited number of Comparative CT (relative quantitation) files to one study
• Group samples and view data both by technical replicate group and biological replicate group
• Use any gene(s) as an endogenous control, including averaging multiple controls together
• Enter known efficiency values to be factored into the RQ results
21 CFR Part 11 Module available
The SDS v1.4 21CFRp11 Module is a powerful tool for assisting with 21CFRp11 compliance, while still offering the flexibility of user-customizable configuration settings.
• Individual user log-ins can be added for up to four user groups, each group with designated permission settings
• User-customizable permission settings include fourteen system activities, e-signature authority designation, and additional security settings to give you maximum control over your compliance efforts
• Audit trails can be enabled or disabled depending on your traceability needs
• A selection of e-signatures is available to ease e-signatures into your workflow
Supports Many Applications
Applications include gene expression analysis, pathogen quantitation, SNP genotyping, isothermal and +⁄– assays utilizing internal positive controls. To facilitate many of these applications, Applied Biosystems® provides preformulated, ready-to-use, quality-tested, TaqMan® assays for use with the 7500 system. Now you can reduce your assay optimization efforts.
Upgrade to High-Speed Thermal Cycling
An optional upgrade to the 7500 Fast System is available. This 7500 Fast System uses our master mix formulations and enables you to shorten your real-time PCR runs to as little as 30 minutes.
The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This system is a sophisticated platform for users who require extended capabilities and maximum versatility. The 3rd generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.
• Powerful, five-color platform is calibrated for the broadest range of dyes available: FAM™⁄SYBR® Green I, VIC®⁄JOE™, NED™⁄ TAMRA™⁄ Cy3™, ROX™⁄Texas Red®, and Cy5™ dyes
• Specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filter sets
• Advanced multi-componenting algorithm minimizes spectral crosstalk – superior for multiplexing
• User-friendly software includes plate set-up wizards, multi-plate data viewing capabilities, and advanced analysis tools to make data processing simple and straightforward
NEW - 7500 Software v2.0
Now the easy-to-use StepOne software is available for both the 7500 and 7500 Fast systems with the 7500 Software v2.0 upgrade. The 7500 Software v2.0 incorporates your favorite StepOne Software features, such as a variety of plate setup wizards, standard curve dilution and master mix recipe calculators, QC flags, data filters, and email notification when a run is finished. The 7500 Software v2.0 also includes an enhanced Gene Expression Study package and has a variety of new melting curve protocol options, including multiple peak detection, step and hold temperature control, and customizable ramp rates.
The NEW Gene Expression Study package accommodates large studies better than any other instrument software package:
• Import an unlimited number of Comparative CT (relative quantitation) files to one study
• Group samples and view data both by technical replicate group and biological replicate group
• Use any gene(s) as an endogenous control, including averaging multiple controls together
• Enter known efficiency values to be factored into the RQ results
21 CFR Part 11 Module available
The SDS v1.4 21CFRp11 Module is a powerful tool for assisting with 21CFRp11 compliance, while still offering the flexibility of user-customizable configuration settings.
• Individual user log-ins can be added for up to four user groups, each group with designated permission settings
• User-customizable permission settings include fourteen system activities, e-signature authority designation, and additional security settings to give you maximum control over your compliance efforts
• Audit trails can be enabled or disabled depending on your traceability needs
• A selection of e-signatures is available to ease e-signatures into your workflow
Supports Many Applications
Applications include gene expression analysis, pathogen quantitation, SNP genotyping, isothermal and +⁄– assays utilizing internal positive controls. To facilitate many of these applications, Applied Biosystems® provides preformulated, ready-to-use, quality-tested, TaqMan® assays for use with the 7500 system. Now you can reduce your assay optimization efforts.
Upgrade to High-Speed Thermal Cycling
An optional upgrade to the 7500 Fast System is available. This 7500 Fast System uses our master mix formulations and enables you to shorten your real-time PCR runs to as little as 30 minutes.
? 功能強大的五色平臺已經過校準，支持較多種類的染料：FAM™/SYBR® Green I、VIC®/JOE™、ED™/TAMRA™/Cy®3、ROX™/Texas Red® 和 Cy®5 染料Applied Biosystems® 7500 實時熒光定量 PCR 系統為要求較大的染料功能多樣性的實驗室提供了一種可靠的平臺。該平臺具有靈敏的光學系統，適用于寬廣光譜范圍內的多種熒光染料。可變的激發波長可提高長波長 (紅色) 染料的檢測靈敏度。
7500 軟件
7500 軟件融合了多種易于使用的特性，如各種反應板設置向導、標準曲線稀釋和預混液配方計算器、QC標記、數據過濾器，并可在運行完成時通過電子郵件通知。7500 軟件還包括功能強大的基因表達研究軟件包，并提供各種熔解曲線實驗方案選項，包括多峰檢測、步進式溫度控制和可定制的變溫速率。可提供遵循美國聯邦法規第 21 章第 11 節 (21 CFR Part 11) 的模塊SDS v1.4 21CFR Part 11 模塊是一款功能強大的工具，它遵循美國聯邦法規第 21 章第 11 節的要求，同時還可提供用戶可定制的配置設置靈活性。
支持多種應用
其應用領域包括基因表達分析、病原體定量、SNP 基因分型、拷貝數變異、恒溫和采用內部陽性對照的 +/–Assay。為方便上述應用領域，Life Technologies 提供了預先配制、已經過質量測試的即用型 TaqMan® Assay，可與 7500 系統配合使用。現在您即可節省在分析優化方面的精力。
升級至 7500 Fast 系統
您可以選擇升級至 7500 Fast 系統。該 7500 Fast 系統采用了我們的預混液配方，使您可以將實時熒光定量 PCR 程序縮短至 30 分鐘。
7500 系統包括：
? 可選擇 Dell® 商用系列筆記本或配有平板顯示器的臺式電腦
? 化學安裝試劑盒包括染料校正反應板、RNA酶 P 儀器驗證反應板和應用特異的 starter kit
? 儀器操作和分析軟件，以及 Primer Express®引物和探針設計軟件
? 包括入門指南在內的豐富的文件集，適用于各種主要的實時熒光定量 PCR 應用領域
? 由 Life Technologies 服務工程師提供的安裝服務
7500 軟件
7500 軟件融合了多種易于使用的特性，如各種反應板設置向導、標準曲線稀釋和預混液配方計算器、QC標記、數據過濾器，并可在運行完成時通過電子郵件通知。7500 軟件還包括功能強大的基因表達研究軟件包，并提供各種熔解曲線實驗方案選項，包括多峰檢測、步進式溫度控制和可定制的變溫速率。可提供遵循美國聯邦法規第 21 章第 11 節 (21 CFR Part 11) 的模塊SDS v1.4 21CFR Part 11 模塊是一款功能強大的工具，它遵循美國聯邦法規第 21 章第 11 節的要求，同時還可提供用戶可定制的配置設置靈活性。
支持多種應用
其應用領域包括基因表達分析、病原體定量、SNP 基因分型、拷貝數變異、恒溫和采用內部陽性對照的 +/–Assay。為方便上述應用領域，Life Technologies 提供了預先配制、已經過質量測試的即用型 TaqMan® Assay，可與 7500 系統配合使用。現在您即可節省在分析優化方面的精力。
升級至 7500 Fast 系統
您可以選擇升級至 7500 Fast 系統。該 7500 Fast 系統采用了我們的預混液配方，使您可以將實時熒光定量 PCR 程序縮短至 30 分鐘。
7500 系統包括：
? 可選擇 Dell® 商用系列筆記本或配有平板顯示器的臺式電腦
? 化學安裝試劑盒包括染料校正反應板、RNA酶 P 儀器驗證反應板和應用特異的 starter kit
? 儀器操作和分析軟件，以及 Primer Express®引物和探針設計軟件
? 包括入門指南在內的豐富的文件集，適用于各種主要的實時熒光定量 PCR 應用領域
? 由 Life Technologies 服務工程師提供的安裝服務
For Research Use Only. Not for use in diagnostic procedures.
7500實時熒光定量PCR系統規格參數：
| Format: | 96-well plate |
| Optics: | Tungsten-halogen lamp, 5 emission filters, CCD Camera, 5 excitation filters |
| Weight: | 75 lbs |
| Precision: | 99.7% confidence (5000 & 10000 copies) |
| Dimensions: | Width: 34 cm (13.39 in), Height: 49 cm (19.29 in), Depth: 45 cm (17.72 in) |
| Throughput: | 4 to 5-96-well plates per 8-hr day |
| Power Limit: | 950 W |
| Sensitivity: | 1 copy of RNase P gene (human genomic DNA) |
| Product Size: | 1 system |
| Dynamic Range: | 9 logs |
| Input Voltage: | 120V |
| Calibrated Dye: | SYBR® Green I, Cy3™, Cy5™, FAM™, JOE™, VIC™, Texas Red®, TAMRA™, ROX™, NED™ |
| Reaction Speed: | Standard |
| Sample Ramp Rate: | ± 1.1°C⁄sec |
| External Computer: | Notebook: Intel Core 2 Duo T5500 (1.66 GHz), 80 GB HD, 8X DVD, 1.0 G DDR2-533 MHz SDRAM |
| Peak Block Ramp Rate: | 2.5°C⁄sec |
| Temperature Accuracy: | 0.25°C (35°C to 95°C after 3 min) |
| Passive Reference Dye: | ROX (Separate Tube), No ROX, ROX (Pre-mixed) |
| Reaction Volume Range: | 20-100 µl |
| Software Compatibility: | Primer Express, 7500 System Software (RQ included), 21 CFR Part 11 Module (optional) |
| Temperature Uniformity: | 0.50°C (after 30 sec) |
| Thermal Cycling System: | Peltier-Based System |
| For Use With (Equipment): | 7500 System |
| Temperature Range (Metric): | 4-100°C |
| High Throughput Compatibility: | Multiplexing |
7500 實時熒光定量PCR系統訂購信息
| 產品名稱 | 訂貨編號 | 數量單位 |
| 7500 Fast System Upgrade Kit | 4362143 | 1 臺儀器 |
| 7500 Real-Time PCR System with Dell™ Notebook | 4351104 | 1 臺儀器 |
| 7500 Real-Time PCR System with Dell™ Tower | 4351105 | 1 臺儀器 |
| Notebook Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems | 4359286 | 1 套 |
| Tower Computer Kit for 7300/7500/7500 Fast Real-Time PCR Systems | 4359284 | 1 套 |
| 7500 System SDS v1.4 21 CFR Part 11 Module | 4377354 | 1 套 |
7500熒光定量PCR儀訂購信息
| 產品描述 | 貨號 | 數量 |
| 7500實時熒光定量PCR擴增儀（配備筆記本電腦） | 4351104 | 1臺 |
| 7500實時熒光定量PCR擴增儀（配備臺式電腦） | 4351105 | 1臺 |
| 7500實時熒光定量PCR擴增儀快速模式升級 | 4362143 | 1個 |
| 7500實時熒光定量PCR擴增儀SDS軟件21 CFR Part 11 模塊 | 4377354 | 1個 |
7500Fast熒光定量PCR儀訂購信息
| 產品描述 | 貨號 | 數量 |
| 新型高分辨率熔解曲線(HRM)軟件 | 4397808 | 1套 |
| 7500快速實時熒光定量PCR擴增儀（配備筆記本電腦） | 4351106 | 1臺 |
| 7500快速實時熒光定量PCR擴增儀（配備臺式電腦） | 4351107 | 1臺 |
| 7500實時熒光定量PCR擴增儀快速模式升級 | 4362143 | 1臺 |
| 7500快速實時熒光定量PCR擴增儀SDS軟件21 CFR Part 11 模塊 | 4377355 | 1套 |
